Genome sequencing

Genome Sequencing

The concept of genome sequencing is quite simple. Break your genome up into many different small fragments, clone those fragments into a cloning vector, isolate many clones, and sequence each clone. All of the techniques used for sequencing are well established. These are the same techniques that scientists used for the past twenty years to characterize many different individual genes. Using th...

Whole Genome Sequencing: An Efficient Approach in Food Safety Management System

Comparing whole genome sequencing and exome sequencing

Introduction Next-generation DNA sequencing empowers scientists to identify genetic variations associated with human disease at higher resolution and greater sensitivity than previously possible. Two approaches are commonly employed -exome sequencing and whole genome sequencing. Exome sequencing targets protein-coding regions comprising approximately 1% of the human genome, while whole genome s...

Sequencing a Microbial Genome

Although many genomes are available for download today, the underlying technologies should not be taken for granted. By using shotgun sequencing techniques and a gauntlet of informatics, we are able to produce high-quality DNA sequence. We will first look at some of the robotics and chemistries of preparing DNA as samples for the sequencing instruments. Then we will look at the series of applic...

Whole-Genome Bisulfite Sequencing

Next-generation sequencing technology has enabled genome-wide analysis of 5mC nucleotides at single-nucleotide resolution. Previous methods for whole-genome bisulfite sequencing (WGBS) yielded reduced genomic representation due to required DNA shearing, ligation of methylated sequencing adapters, and bisulfite conversion of unmethylated cytidine residues prior to sequencing. One disadvantage of...